Non-Enzymatic Modification of Aminophospholipids by Carbonyl-Amine Reactions

Non-Enzymatic Modification of Aminophospholipids by Carbonyl-Amine Reactions

Non-enzymatic modification of aminophospholipids by lipid peroxidation-derived aldehydes and reducing sugars through carbonyl-amine reactions are thought to contribute to the age-related deterioration of cellular membranes and to the pathogenesis of diabetic complications. Much evidence demonstrates the modification of aminophospholipids by glycation, glycoxidation and lipoxidation reactions. ...

Fluorescent pyrrole products from carbonyl-amine reactions.

The reaction of (E)-4,5-epoxy-(E)-2-heptenal with butylamine and glycine methyl ester produced brown macromolecular pigments, which showed fluorescent characteristics similar to lipofuscins. This polymerization reaction implies in the first step the formation of 1-alkyl-2-(1'-hydroxyalkyl)pyrroles (IV, VI). Compounds IV and VI have been isolated and characterized from the above reactions, and t...

Protective effect of phenolic compounds on carbonyl-amine reactions

Post-translational non-enzymatic modification of proteins. II. Separation of selected protein species after glycation and other carbonyl-mediated modifications.

There are two strategies applicable to revealing non-enzymatic post-translational modifications of proteins; while assaying of the hydrolytically stable adducts was the subject of our previous communication [1], here we attempted to review separation technologies for the unfragmented modified proteins. There are a few standard procedures used for this purpose, namely Laemmli gel electrophoresis...

Organic reactions catalyzed by rhenium carbonyl complexes.